Reporter

Part:BBa_K4799602:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


NtNRAMP2_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal SpeI site found at 2227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 4036
    Illegal SpeI site found at 2227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 2018
    Illegal BamHI site found at 2001
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal SpeI site found at 2227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal SpeI site found at 2227
    Illegal NgoMIV site found at 2451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2251
    Illegal BsaI.rc site found at 2866
    Illegal SapI.rc site found at 3037


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References